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Although several clinical options for healing such defects exist, all have major disadvantages. We describe a novel approach to bone regeneration using chemically modified messenger RNA (cmRNA). An optimized cmRNA encoding bone morphogenetic protein 2 (BMP-2) was delivered to critical size, mid-diaphyseal, femoral osteotomies in rats. The cmRNA remained orthotopically localized and generated BMP locally for several days. Defects healed at doses > 25 µg BMP-2 cmRNA. By four weeks, all animals treated with 50 µg BMP-2 cmRNA had bridged bone defects without forming the massive callus seen with rhBMP-2. Transcriptomic analysis and immunohistochemistry confirmed that cmRNA regenerated bone via endochondral ossification, whereas rhBMP-2 drove intramembranous bone formation. The osteogenic properties of the optimized cmRNA were superior to those of rhBMP-2 and provide the basis of an innovative, safe and highly translatable technology for healing osseous defects."},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2021-05-27"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"Hebels, Dennie"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2021-05-27"}]},"dansDataVaultMetadata":{"displayName":"Data Vault Metadata","name":"dansDataVaultMetadata","fields":[{"typeName":"dansDataversePid","multiple":false,"typeClass":"primitive","value":"doi:10.34894/XUOHLG"},{"typeName":"dansDataversePidVersion","multiple":false,"typeClass":"primitive","value":"1.1"},{"typeName":"dansBagId","multiple":false,"typeClass":"primitive","value":"urn:uuid:fd1b9d3f-d382-4a85-8e07-64c3cd2492a7"},{"typeName":"dansNbn","multiple":false,"typeClass":"primitive","value":"urn:nbn:nl:ui:13-adab9f82-f647-4a4b-bbb9-56f37e2bd9bc"}]}},"files":[{"label":"Supporting Dataset_De la Vega et al.pdf","restricted":false,"version":1,"datasetVersionId":30251,"dataFile":{"id":195143,"persistentId":"","filename":"Supporting Dataset_De la Vega et al.pdf","contentType":"application/pdf","friendlyType":"Adobe PDF","filesize":121780,"storageIdentifier":"surf://store:17c9c88b5ce-2f95946f4f1c","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"344089118e206e4fb86a58c5c34fdf83e4b04ae4"},"tabularData":false,"creationDate":"2021-10-20","publicationDate":"2022-12-12","lastUpdateTime":"2025-02-18T16:23:02Z","fileAccessRequest":false}}]}}}