{"status":"OK","data":{"id":106319,"identifier":"XPIIU6","persistentUrl":"https://doi.org/10.34894/XPIIU6","protocol":"doi","authority":"10.34894","separator":"/","publisher":"DataverseNL","publicationDate":"2021-09-03","storageIdentifier":"surf://10.34894/XPIIU6","effectiveDatasetFileCountLimit":10000,"datasetFileUploadsAvailable":9995,"datasetType":"dataset","locks":[],"latestVersion":{"id":9345,"datasetId":106319,"datasetPersistentId":"doi:10.34894/XPIIU6","datasetType":"dataset","storageIdentifier":"surf://10.34894/XPIIU6","versionNumber":1,"internalVersionNumber":8,"versionMinorNumber":2,"versionState":"RELEASED","latestVersionPublishingState":"RELEASED","deaccessionLink":"","lastUpdateTime":"2021-11-17T16:30:33Z","releaseTime":"2021-11-17T16:30:33Z","createTime":"2021-11-17T16:25:33Z","publicationDate":"2021-09-03","citationDate":"2021-09-03","effectiveDatasetFileCountLimit":10000,"datasetFileUploadsAvailable":9995,"termsOfUse":"<a rel=\"license\" href=\"http://creativecommons.org/licenses/by/4.0/\"><img alt=\"Creative Commons License\" style=\"border-width:0\" src=\"https://i.creativecommons.org/l/by/4.0/88x31.png\" /></a><br />This work is licensed under a <a rel=\"license\" href=\"http://creativecommons.org/licenses/by/4.0/\">Creative Commons Attribution 4.0 International License</a>.","termsOfAccess":"Made publicly available upon publication of paper","fileAccessRequest":true,"metadataBlocks":{"citation":{"displayName":"Citation Metadata","name":"citation","fields":[{"typeName":"title","multiple":false,"typeClass":"primitive","value":"Circulating microRNAs in dogs with Cushing's syndrome"},{"typeName":"author","multiple":true,"typeClass":"compound","value":[{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Sanders, Karin"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"0000-0001-9634-9853"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Veldhuizen, Anouk"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Kooistra, Hans S."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Slob, Adri"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Timmermans-Sprang, Elpetra P.M."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Riemers, Frank M."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Daminet, Sylvie"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Ghent University"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Fracassi, Federico"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"University of Bologna"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Van Nimwegen, Sebastiaan A."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Meij, Björn P."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Galac, Sara"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}}]},{"typeName":"datasetContact","multiple":true,"typeClass":"compound","value":[{"datasetContactName":{"typeName":"datasetContactName","multiple":false,"typeClass":"primitive","value":"Sanders, Karin"},"datasetContactAffiliation":{"typeName":"datasetContactAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}}]},{"typeName":"dsDescription","multiple":true,"typeClass":"compound","value":[{"dsDescriptionValue":{"typeName":"dsDescriptionValue","multiple":false,"typeClass":"primitive","value":"Canine Cushing’s syndrome (hypercortisolism) can be caused by a pituitary tumor (pituitary-dependent hypercortisolism; PDH) or a cortisol-secreting adrenocortical tumor (csACT). For both cases, noninvasive biomarkers that could pre-operatively predict the risk of recurrence after surgery would greatly impact clinical decision making. The aim of this study was to determine whether circulating microRNAs (miRNAs) can be used as noninvasive biomarkers for canine Cushing’s syndrome. \n\nAfter a pilot study with 40 miRNAs in blood samples of healthy dogs (n = 3), dogs with PDH (n = 3) and dogs with a csACT (n = 4), we selected a total of 20 miRNAs for the definitive study. In the definitive study, these 20 miRNAs were analyzed in blood samples of healthy dogs (n = 6), dogs with PDH (n = 19, pre- and post-operative samples) and dogs with a csACT (n = 26, pre-operative samples). \n\nIn dogs with PDH, six miRNAs (miR-122-5p, miR-126-5p, miR-141-3p, miR-222-3p, miR-375-3p and miR-483-3p) were differentially expressed compared to healthy dogs. Of one miRNA, miR-122-5p, the expression levels did not overlap between healthy dogs and dogs with PDH (p = 2.9x10-4), significantly decreased after hypophysectomy (p = 0.013), and were significantly higher (p = 0.017) in dogs with recurrence (n = 3) than in dogs without recurrence for at least one year after hypophysectomy (n = 7). In dogs with csACTs, two miRNAs (miR-483-3p and miR-223-3p) were differentially expressed compared to healthy dogs. Additionally, miR-141-3p was expressed significantly lower (p = 0.009) in dogs with csACTs that had a histopathological Utrecht score of ≥ 11 compared to those with a score of < 11.\n\nThese results indicate that circulating miRNAs have the potential to be noninvasive biomarkers in dogs with Cushing’s syndrome that may contribute to clinical decision-making."}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"keyword","multiple":true,"typeClass":"compound","value":[{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"miRNA"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"pituitary-dependent hypercortisolism"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Cushing's disease"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"adrenocortical tumor"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"miR-122"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"prognostic"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"recurrence"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"normalization"}}]},{"typeName":"publication","multiple":true,"typeClass":"compound","value":[{"publicationCitation":{"typeName":"publicationCitation","multiple":false,"typeClass":"primitive","value":"Karin Sanders, Anouk Veldhuizen, Hans S. Kooistra, Adri Slob, Elpetra P.M. Timmermans-Sprang, Frank M. Riemers, Sylvie Daminet, Federico Fracassi, Sebastiaan A. van Nimwegen, Björn P. Meij, Sara Galac (2021) <b>Circulating MicroRNAs as Non-invasive Biomarkers for Canine Cushing’s Syndrome.</b>\n<i>Front. Vet. Sci. </i>8:760487."},"publicationIDType":{"typeName":"publicationIDType","multiple":false,"typeClass":"controlledVocabulary","value":"doi"},"publicationIDNumber":{"typeName":"publicationIDNumber","multiple":false,"typeClass":"primitive","value":"10.3389/fvets.2021.760487"},"publicationURL":{"typeName":"publicationURL","multiple":false,"typeClass":"primitive","value":"https://doi.org/10.3389/fvets.2021.760487"}},{"publicationCitation":{"typeName":"publicationCitation","multiple":false,"typeClass":"primitive","value":"Karin Sanders, Anouk Veldhuizen, Hans S. Kooistra, Adri Slob, Elpetra P.M. Timmermans-Sprang, Frank M. Riemers, Sylvie Daminet, Federico Fracassi, Sebastiaan A. van Nimwegen, Björn P. Meij, Sara Galac (2021) <b>Circulating microRNAs as noninvasive biomarkers for canine Cushing’s syndrome</b>\n<i>bioRxiv</i> 2021.08.17.456598."},"publicationIDType":{"typeName":"publicationIDType","multiple":false,"typeClass":"controlledVocabulary","value":"doi"},"publicationIDNumber":{"typeName":"publicationIDNumber","multiple":false,"typeClass":"primitive","value":"10.1101/2021.08.17.456598"},"publicationURL":{"typeName":"publicationURL","multiple":false,"typeClass":"primitive","value":"https://doi.org/10.1101/2021.08.17.456598"}}]},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"Sanders, Karin"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2021-07-20"}]},"dansDataVaultMetadata":{"displayName":"Data Vault Metadata","name":"dansDataVaultMetadata","fields":[{"typeName":"dansDataversePid","multiple":false,"typeClass":"primitive","value":"doi:10.34894/XPIIU6"},{"typeName":"dansDataversePidVersion","multiple":false,"typeClass":"primitive","value":"1.2"},{"typeName":"dansBagId","multiple":false,"typeClass":"primitive","value":"urn:uuid:653f3d29-7be9-4b4f-8321-5535b05e8f39"},{"typeName":"dansNbn","multiple":false,"typeClass":"primitive","value":"urn:nbn:nl:ui:13-90296c80-7eef-47f7-908c-84532ed8fe01"}]}},"files":[{"label":"MiRNA_Assay lists.xlsx","restricted":false,"version":1,"datasetVersionId":9345,"dataFile":{"id":110382,"persistentId":"","filename":"MiRNA_Assay lists.xlsx","contentType":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","friendlyType":"MS Excel Spreadsheet","filesize":18898,"storageIdentifier":"surf://store:17b5331a8b0-d66d834804b4","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"c884e43d130c71e3a36675b289cf85b453704497"},"tabularData":false,"creationDate":"2021-08-17","publicationDate":"2021-09-03","lastUpdateTime":"2021-11-17T16:30:33Z","fileAccessRequest":true}},{"label":"MiRNA_Clinical data.xlsx","restricted":false,"version":1,"datasetVersionId":9345,"dataFile":{"id":110381,"persistentId":"","filename":"MiRNA_Clinical data.xlsx","contentType":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","friendlyType":"MS Excel Spreadsheet","filesize":14752,"storageIdentifier":"surf://store:17b5331a94a-3555845ecf77","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"861eef265a7762bd8447e5ff2d4fd6cf00d66db9"},"tabularData":false,"creationDate":"2021-08-17","publicationDate":"2021-09-03","lastUpdateTime":"2021-11-17T16:30:33Z","fileAccessRequest":true}},{"label":"MiRNA_Definitive study results.xlsx","restricted":false,"version":1,"datasetVersionId":9345,"dataFile":{"id":110379,"persistentId":"","filename":"MiRNA_Definitive study results.xlsx","contentType":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","friendlyType":"MS Excel Spreadsheet","filesize":108161,"storageIdentifier":"surf://store:17b5331aa03-72b2ef73bf64","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"8c5d6a420e654ae2c19c29de2919a6825bc21518"},"tabularData":false,"creationDate":"2021-08-17","publicationDate":"2021-09-03","lastUpdateTime":"2021-11-17T16:30:33Z","fileAccessRequest":true}},{"label":"MiRNA_Pilot study results.xlsx","restricted":false,"version":1,"datasetVersionId":9345,"dataFile":{"id":110380,"persistentId":"","filename":"MiRNA_Pilot study results.xlsx","contentType":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","friendlyType":"MS Excel Spreadsheet","filesize":27756,"storageIdentifier":"surf://store:17b5331aa70-5efbb4684999","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"0818f9923bcf24186d31f11d7883946f1e92d11f"},"tabularData":false,"creationDate":"2021-08-17","publicationDate":"2021-09-03","lastUpdateTime":"2021-11-17T16:30:33Z","fileAccessRequest":true}},{"label":"README.txt","restricted":false,"version":1,"datasetVersionId":9345,"dataFile":{"id":110378,"persistentId":"","filename":"README.txt","contentType":"text/plain","friendlyType":"Plain Text","filesize":4268,"storageIdentifier":"surf://store:17b532e75fa-db151dffa0d7","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"bda74c404a0a60c369a6245fdee71a1d11ef94cf"},"tabularData":false,"creationDate":"2021-08-17","publicationDate":"2021-09-03","lastUpdateTime":"2021-11-17T16:30:33Z","fileAccessRequest":true}}]}}}