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output files from the TECAN plate reader used in the in the publication with the title: “Structural and functional implications of phase separation of membrane protein LacY in Escherichia coli “.<br>\nFiles are organized based on the figure they were used in. Molecular dynamic simulations input files, trajectories and code for the analysis are available on Zenodo: 10.5281/zenodo.17335657.\n<br>"}},{"dsDescriptionValue":{"typeName":"dsDescriptionValue","multiple":false,"typeClass":"primitive","value":"Liquid-liquid phase-separation (LLPS) controls protein activity and dynamically organizes (macro)molecules in living systems without the need for membrane-bound compartments. Biomolecular condensates of water-soluble proteins have extensively been studied, but little is known about LLPS of membrane proteins. In this work we induce in vivo condensation of lactose permease (LacY), a widely-studied model monomeric inner membrane protein in Escherichia coli, and evaluate how it affects LacY function. We fused LacY with engineered, condensate-forming protein PopTag. We observe major changes in the localization and mobility of LacY-Pop. Molecular dynamics simulations show how the PopTag domain drives the condensate-like association dynamics of LacY-Pop through hydrophobic sticker interactions. LacY-Pop preserves native-level transport activity and outperforms the non-condensed LacY under mild hyperosmotic stress (osmotic upshift). In osmotically stressed cells, membrane-bound biomolecular condensates also reduce deformation of the cytoplasmic membrane. Perturbation experiments suggest that membrane curvature drives the accumulation of LacY-Pop at the poles of E. coli. Co-condensation of LacY and β-galactosidase LacZ slightly reduces their activity and results in remarkable cellular reorganization of the proteins. Our research shows the localization, dynamics, and function of phase-separated membrane proteins in bacteria and highlights the potential of LLPS for engineering complex metabolic networks in vivo."},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2026"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"keyword","multiple":true,"typeClass":"compound","value":[{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Liquid-liquid phase-separation"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Membrane proteins"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Biomolecular condensates"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Single-molecule displacement mapping (SMdM)"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Photoactivated localization microscopy (PALM)"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Enzymatic pathway"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Engineered in vivo condensates"}}]},{"typeName":"publication","multiple":true,"typeClass":"compound","value":[{"publicationRelationType":{"typeName":"publicationRelationType","multiple":false,"typeClass":"controlledVocabulary","value":"IsSupplementTo"},"publicationCitation":{"typeName":"publicationCitation","multiple":false,"typeClass":"primitive","value":"Linnik, D. et al. 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Downloading the data has to be done in sections of approx. 9 GB each."},{"typeName":"language","multiple":true,"typeClass":"controlledVocabulary","value":["English"]},{"typeName":"distributor","multiple":true,"typeClass":"compound","value":[{"distributorName":{"typeName":"distributorName","multiple":false,"typeClass":"primitive","value":"DataverseNL network"},"distributorAffiliation":{"typeName":"distributorAffiliation","multiple":false,"typeClass":"primitive","value":"DANS"},"distributorAbbreviation":{"typeName":"distributorAbbreviation","multiple":false,"typeClass":"primitive","value":"DVNL"},"distributorURL":{"typeName":"distributorURL","multiple":false,"typeClass":"primitive","value":"https://dataverse.nl/"}}]},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"Silva Correia Alves, Ana"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2026-01-28"},{"typeName":"relatedDatasets","multiple":true,"typeClass":"primitive","value":["Stevens, J. 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