{"status":"OK","data":{"id":488599,"identifier":"UAORA8","persistentUrl":"https://doi.org/10.34894/UAORA8","protocol":"doi","authority":"10.34894","separator":"/","publisher":"DataverseNL","publicationDate":"2025-06-26","storageIdentifier":"surf://10.34894/UAORA8","effectiveDatasetFileCountLimit":10000,"datasetFileUploadsAvailable":9999,"datasetType":"dataset","locks":[],"latestVersion":{"id":30767,"datasetId":488599,"datasetPersistentId":"doi:10.34894/UAORA8","datasetType":"dataset","storageIdentifier":"surf://10.34894/UAORA8","versionNumber":1,"internalVersionNumber":12,"versionMinorNumber":1,"versionState":"RELEASED","latestVersionPublishingState":"RELEASED","deaccessionLink":"","lastUpdateTime":"2025-07-08T07:21:34Z","releaseTime":"2025-07-08T07:21:34Z","createTime":"2025-06-26T08:20:57Z","publicationDate":"2025-06-26","citationDate":"2025-06-26","effectiveDatasetFileCountLimit":10000,"datasetFileUploadsAvailable":9999,"license":{"name":"CC0-1.0","uri":"http://creativecommons.org/publicdomain/zero/1.0","iconUri":"https://licensebuttons.net/p/zero/1.0/88x31.png","rightsIdentifier":"CC0-1.0","rightsIdentifierScheme":"SPDX","schemeUri":"https://spdx.org/licenses/","languageCode":"en"},"fileAccessRequest":true,"metadataBlocks":{"citation":{"displayName":"Citation Metadata","name":"citation","fields":[{"typeName":"title","multiple":false,"typeClass":"primitive","value":"Supplementary Data for \"Canine adrenomedullary and pheochromocytoma organoids: a novel in vitro model\""},{"typeName":"author","multiple":true,"typeClass":"compound","value":[{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"M.F. van den Berg"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"https://ror.org/04pp8hn57","expandedvalue":{"scheme":"http://www.grid.ac/ontology/","termName":"Utrecht University","@type":"https://schema.org/Organization"}},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"0000-0002-2016-119X"}}]},{"typeName":"datasetContact","multiple":true,"typeClass":"compound","value":[{"datasetContactName":{"typeName":"datasetContactName","multiple":false,"typeClass":"primitive","value":"van den Berg, Marit"},"datasetContactAffiliation":{"typeName":"datasetContactAffiliation","multiple":false,"typeClass":"primitive","value":"Utrecht University"}}]},{"typeName":"dsDescription","multiple":true,"typeClass":"compound","value":[{"dsDescriptionValue":{"typeName":"dsDescriptionValue","multiple":false,"typeClass":"primitive","value":"Supplementary Data for \"Canine adrenomedullary and pheochromocytoma organoids: a novel in vitro model\". \nvan den Berg MF, Timmermans-Sprang EPM, Viets FC, van den Berg L, Danawar F, van Wolferen ME, Kooistra HS, Grinwis GCM, de Jong WHA, van Faassen M, Galac S.\n\nAbstract\n\nBackground\nGiven the lack of effective medical treatment for pheochromocytomas (PCCs), a reliable in vitro model is needed to explore new therapies. Organoids are three-dimensional self-renewing structures that exhibit key features of their tissue of origin, providing valuable platforms for disease modeling and drug screening.\n\nAim\nThis study aimed to establish and characterize organoid cultures of canine normal adrenal medullas and PCCs.\n\nMethods\nNormal adrenal medullas from healthy dogs and tumor tissue from client-owned dogs with PCC were used to develop organoids. Primary cell suspensions were cultured in a 3D matrix, and organoids were established under optimized conditions. Organoids were characterized using histology, immunohistochemistry, immunofluorescence, qPCR, and metanephrine analysis by LC-MS/MS.\n\nResults\nFive adrenomedullary organoid lines were successfully established, demonstrating sustained growth. Organoid cultures were also derived from nine PCCs, although expansion was limited after passages 1-2. Both adrenomedullary and PCC organoids expressed differentiation markers (chromogranin A, synaptophysin, phenylethanolamine N-methyltransferase) and stem/progenitor markers (nestin, SOX10). Organoids retained key functional traits, as indicated by metanephrine levels in culture supernatants, which initially mirrored primary tumor patterns. A decline in both differentiation marker expression and metanephrine levels was observed over time, possibly due to organoid dedifferentiation or selective loss of differentiated chromaffin cells.\n\nConclusions\nThis study demonstrates the establishment of the first adrenomedullary and PCC organoid lines. While further optimization is needed, these organoids offer valuable potential as an in vitro model to investigate PCC pathophysiology and explore novel treatment strategies for this therapeutically challenging tumor."},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2025-06-26"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"publication","multiple":true,"typeClass":"compound","value":[{"publicationRelationType":{"typeName":"publicationRelationType","multiple":false,"typeClass":"controlledVocabulary","value":"IsSupplementTo"},"publicationCitation":{"typeName":"publicationCitation","multiple":false,"typeClass":"primitive","value":"\"Canine adrenomedullary and pheochromocytoma organoids: a novel in vitro model\". \nvan den Berg MF, Timmermans-Sprang EPM, Viets FC, van den Berg L, Danawar F, van Wolferen ME, Kooistra HS, Grinwis GCM, de Jong WHA, van Faassen M, Galac S."},"publicationIDType":{"typeName":"publicationIDType","multiple":false,"typeClass":"controlledVocabulary","value":"doi"},"publicationIDNumber":{"typeName":"publicationIDNumber","multiple":false,"typeClass":"primitive","value":"10.1210/endocr/bqaf114"},"publicationURL":{"typeName":"publicationURL","multiple":false,"typeClass":"primitive","value":"https://doi.org/10.1210/endocr/bqaf114"}}]},{"typeName":"grantNumber","multiple":true,"typeClass":"compound","value":[{"grantNumberAgency":{"typeName":"grantNumberAgency","multiple":false,"typeClass":"primitive","value":"Maria Naundorf van Gorkum Foundation"}},{"grantNumberAgency":{"typeName":"grantNumberAgency","multiple":false,"typeClass":"primitive","value":"Foundation Abri"}},{"grantNumberAgency":{"typeName":"grantNumberAgency","multiple":false,"typeClass":"primitive","value":"Friends of VetMed"}}]},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"van den Berg, Marit"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2025-06-26"}]},"dansDataVaultMetadata":{"displayName":"Data Vault Metadata","name":"dansDataVaultMetadata","fields":[{"typeName":"dansDataversePid","multiple":false,"typeClass":"primitive","value":"doi:10.34894/UAORA8"},{"typeName":"dansDataversePidVersion","multiple":false,"typeClass":"primitive","value":"1.1"},{"typeName":"dansBagId","multiple":false,"typeClass":"primitive","value":"urn:uuid:f6e1e45b-ac71-4f9e-8e18-538f62e92732"},{"typeName":"dansNbn","multiple":false,"typeClass":"primitive","value":"urn:nbn:nl:ui:13-951f395c-0d3a-42e8-b23e-05cfe901b357"}]}},"files":[{"label":"Supplementary data.pdf","restricted":false,"version":1,"datasetVersionId":30767,"dataFile":{"id":488600,"persistentId":"","filename":"Supplementary data.pdf","contentType":"application/pdf","friendlyType":"Adobe PDF","filesize":1651460,"storageIdentifier":"surf://store:197aaf03f1d-3dd3328b514b","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"e40b437799dde36f85a6fc9379db3cc723d1be96"},"tabularData":false,"creationDate":"2025-06-26","publicationDate":"2025-06-26","lastUpdateTime":"2025-07-08T07:21:34Z","fileAccessRequest":true}}]}}}