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Pediatric autoimmune uveitis is associated with intraocular antibodies against Epstein-Barr virus Nuclear Antigen 1 (EBNA-1).</i>\n<br><br>\n\n\n<b>Raw IgG intensity data (all samples combined and calculated mean per peptide)</b><br>\n<a href=\"https://dataverse.nl/api/access/datafile/449756\" >\"Raw_data_microarrays.csv\"</a>\n<br><br>\n<b>R scripts with step by step analysis</b>\n<br>\n'Statistical analysis' (see <a href=\"https://dataverse.nl/api/access/datafile/449759\">Analysis.html</a>)\n<br>\n'Figure 1' (see <a href=\"https://dataverse.nl/api/access/datafile/449647\" >\"Figure_1.html\"</a>)\n<br>\n'Figure 2 and 3' (see <a href=\"https://dataverse.nl/api/access/datafile/449656\" >\"Figure-2_3.html\"</a>)\n<br><br>\n<b>Infectious disease peptide annotation map</b><br>\n<a href=\"https://dataverse.nl/api/access/datafile/449657\" >\"Peptide_Map_Infectious_Disease_Epitope_Microarray.csv\"</a>"},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2024-08-29"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"keyword","multiple":true,"typeClass":"compound","value":[{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Pediatric uveitis, IgG, antibody, microarray"}}]},{"typeName":"notesText","multiple":false,"typeClass":"primitive","value":"Deep profiling of the antibody-repertoire of patients with non-infectious uveitis may reveal a distinct natural history of microbial exposure that may increase the susceptibility to non-infectious uveitis. This may be reflected by enrichment for IgG antibodies towards distinct pathogenic species or antibody epitope motifs. We used peptide microarrays containing 3,760 linear B-cell epitopes from 196 human pathogens to profile IgG levels in eye fluid biopsies and paired serum samples from 18 Dutch pediatric patients and 6 age-matched controls. We compared intensities of single epitopes and clusters based on overlapping amino acid sequence of peptides. Next-generation sequencing data was obtained to determine the HLA-DRB1*15:01 genotype. Intraocular antibodies against an immunogenic epitope of EBV associated with HLA-DRB1*15:01 is implicated in pediatric uveitis and suggests altered host immune responses to EBV infection in autoimmune uveitis."},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"Hendrikse, Jytte"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2024-08-29"}]},"dansDataVaultMetadata":{"displayName":"Data Vault 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