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Previously, we have demonstrated that a chemically modified messenger RNA (cmRNA) coding for BMP-2 induced rapid and reliable healing until consolidation in a bone segmental defect in rats. Here, we show that the simultaneous transfer of BMP-2 and BMP-7 cmRNAs to stem cells leads to increased osteogenic gene expression, extracellular matrix deposition, and mineralization, compared to single or sequential delivery of these cmRNAs. Using a murine model of ectopic ossification, we found that dual BMP-2/BMP-7 cmRNA delivery using collagen-hydroxyapatite scaffolds, designed specifically for bone repair, ensured local BMP-2 and -7 expression with abundant osteogenesis in the absence of adverse effects. This confirms the superior osteogenic potency achieved by local, controlled dual delivery of cmRNAs encoding BMP-2 and BMP-7, further enhancing the potential of this highly translatable technology for promoting bone healing."},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2025-03-05"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"Hebels, Dennie"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2025-03-05"}]},"dansDataVaultMetadata":{"displayName":"Data Vault Metadata","name":"dansDataVaultMetadata","fields":[{"typeName":"dansDataversePid","multiple":false,"typeClass":"primitive","value":"doi:10.34894/PT4QLL"},{"typeName":"dansDataversePidVersion","multiple":false,"typeClass":"primitive","value":"1.0"},{"typeName":"dansBagId","multiple":false,"typeClass":"primitive","value":"urn:uuid:d24bb0a9-2a7b-4f70-b9f4-28c2978961d4"},{"typeName":"dansNbn","multiple":false,"typeClass":"primitive","value":"urn:nbn:nl:ui:13-5a6d010b-928a-4f71-8bde-69a15a773e8e"}]}},"files":[{"description":"Sequence of investigated chemically modified mRNAs","label":"BMP-2 and -7 cmRNAs.pdf","restricted":false,"version":1,"datasetVersionId":30331,"dataFile":{"id":471704,"persistentId":"","filename":"BMP-2 and -7 cmRNAs.pdf","contentType":"application/pdf","friendlyType":"Adobe PDF","filesize":48093,"description":"Sequence of investigated chemically modified mRNAs","storageIdentifier":"surf://store:19565b82500-77c92d945e57","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"ec93346e2b832502f3a4c6c2683be6d63cb258ef"},"tabularData":false,"creationDate":"2025-03-05","publicationDate":"2025-08-25","lastUpdateTime":"2025-08-25T10:44:56Z","fileAccessRequest":true}},{"label":"Western blots data.pdf","restricted":false,"version":1,"datasetVersionId":30331,"dataFile":{"id":488893,"persistentId":"","filename":"Western blots data.pdf","contentType":"application/pdf","friendlyType":"Adobe PDF","filesize":218653,"storageIdentifier":"surf://store:197e92b41b9-575637a1ae51","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"193681bdf050cc7cdd1dfba47abcffbd92f5cd84"},"tabularData":false,"creationDate":"2025-07-08","publicationDate":"2025-08-25","lastUpdateTime":"2025-08-25T10:44:56Z","fileAccessRequest":true}}]}}}