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Additionally, wide field epifluorescence time lapses were performed to investigate cell proliferation. Various human cell lines (U2OS, HeLa, and RPE-1) and structures (nuclear pore complex, Golgi, mitochondria and actin) were imaged. <br>\nThe dataset contains both long-term (20 h) studies on cell proliferation and survival after high-resolution (50 nm) STED imaging, and the short-term stress response of cells by observing the cytosolic calcium levels during various types of imaging."},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2026"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences","Physics"]},{"typeName":"keyword","multiple":true,"typeClass":"compound","value":[{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"STED microscopy"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"phototoxicity"}}]},{"typeName":"publication","multiple":true,"typeClass":"compound","value":[{"publicationRelationType":{"typeName":"publicationRelationType","multiple":false,"typeClass":"controlledVocabulary","value":"IsCitedBy"},"publicationCitation":{"typeName":"publicationCitation","multiple":false,"typeClass":"primitive","value":"Mol, F.N., Dijt, S.J., Burgers, T.C.Q. et al. 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