{"status":"OK","data":{"id":383843,"identifier":"BG4ZS8","persistentUrl":"https://doi.org/10.34894/BG4ZS8","protocol":"doi","authority":"10.34894","separator":"/","publisher":"DataverseNL","publicationDate":"2024-06-04","storageIdentifier":"surf://10.34894/BG4ZS8","effectiveDatasetFileCountLimit":10000,"datasetFileUploadsAvailable":9989,"datasetType":"dataset","locks":[],"latestVersion":{"id":30477,"datasetId":383843,"datasetPersistentId":"doi:10.34894/BG4ZS8","datasetType":"dataset","storageIdentifier":"surf://10.34894/BG4ZS8","versionNumber":1,"internalVersionNumber":7,"versionMinorNumber":2,"versionState":"RELEASED","latestVersionPublishingState":"RELEASED","deaccessionLink":"","lastUpdateTime":"2025-04-10T12:38:31Z","releaseTime":"2025-04-10T12:38:31Z","createTime":"2025-04-10T12:38:04Z","publicationDate":"2024-06-04","citationDate":"2024-06-04","effectiveDatasetFileCountLimit":10000,"datasetFileUploadsAvailable":9989,"license":{"name":"CC-BY-4.0","uri":"http://creativecommons.org/licenses/by/4.0","iconUri":"https://i.creativecommons.org/l/by/4.0/88x31.png"},"fileAccessRequest":false,"metadataBlocks":{"citation":{"displayName":"Citation Metadata","name":"citation","fields":[{"typeName":"title","multiple":false,"typeClass":"primitive","value":"Replication Data for: Temperature dependence of the mutation rate towards antibiotic resistance"},{"typeName":"author","multiple":true,"typeClass":"compound","value":[{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"van Eldijk, Timo J.B."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Groningen Institute for Evolutionary Life Sciences, University of Groningen"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"0000-0002-2164-1443"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Sheridan, Eleanor A."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Groningen Institute for Evolutionary Life Sciences, University of Groningen"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"0000-0001-7358-7816"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Martin, Guillaume"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Université de Montpellier, CNRS-IRD-EPHE-UM"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"0000-0002-5203-5091"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Weissing, Franz J."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Groningen Institute for Evolutionary Life Sciences, University of Groningen"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"0000-0003-3281-663X"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"Kuipers, Oscar P."},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"0000-0001-5596-7735"}},{"authorName":{"typeName":"authorName","multiple":false,"typeClass":"primitive","value":"van Doorn, G. Sander"},"authorAffiliation":{"typeName":"authorAffiliation","multiple":false,"typeClass":"primitive","value":"Groningen Institute for Evolutionary Life Sciences, University of Groningen"},"authorIdentifierScheme":{"typeName":"authorIdentifierScheme","multiple":false,"typeClass":"controlledVocabulary","value":"ORCID"},"authorIdentifier":{"typeName":"authorIdentifier","multiple":false,"typeClass":"primitive","value":"0000-0002-4703-3788"}}]},{"typeName":"datasetContact","multiple":true,"typeClass":"compound","value":[{"datasetContactName":{"typeName":"datasetContactName","multiple":false,"typeClass":"primitive","value":"van Doorn, G. Sander"},"datasetContactAffiliation":{"typeName":"datasetContactAffiliation","multiple":false,"typeClass":"primitive","value":"Groningen Institute for Evolutionary Life Sciences, University of Groningen"}}]},{"typeName":"dsDescription","multiple":true,"typeClass":"compound","value":[{"dsDescriptionValue":{"typeName":"dsDescriptionValue","multiple":false,"typeClass":"primitive","value":"Environmental conditions can influence mutation rates in bacteria. Fever is a common response to infection that alters the growth conditions of infecting bacteria. Here we examine how a temperature change, such as is associated with fever, affects the mutation rate towards antibiotic resistance.\nWe used a fluctuation test to assess the mutation rate towards antibiotic resistance in Escherichia coli at two different temperatures: 37 °C (normal temperature) and 40 °C (fever temperature). We performed this measurement for three different antibiotics with different modes of action: ciprofloxacin, rifampicin, and ampicillin. In all cases, the mutation rate towards antibiotic resistance turned out to be temperature dependent, but in different ways. Fever temperatures led to a reduced mutation rate towards ampicillin resistance and an elevated mutation rate towards ciprofloxacin and rifampicin resistance.\nThis study opens a new avenue to mitigate the emergence of antibiotic resistance by coordinating the choice of an antibiotic with the decision of whether or not to suppress fever when treating a patient. Hence, optimised combinations of antibiotics and fever suppression strategies may be a new weapon in the battle against antibiotic resistance."},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2023-10-27"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"keyword","multiple":true,"typeClass":"compound","value":[{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"mutation rates"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"antibiotic resistance"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"temperature"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"ciprofloxacin"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"ampicillin"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"rifampicin"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"fever"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"evolutionary medicine"}}]},{"typeName":"publication","multiple":true,"typeClass":"compound","value":[{"publicationCitation":{"typeName":"publicationCitation","multiple":false,"typeClass":"primitive","value":"van Eldijk TJB, Sheridan EA, Martin G, Weissing FJ,  Kuipers OP, van Doorn GS (2024). Temperature dependence of the mutation rate towards antibiotic resistance. JAC-AMR 6(3): dlae085,"},"publicationIDType":{"typeName":"publicationIDType","multiple":false,"typeClass":"controlledVocabulary","value":"doi"},"publicationIDNumber":{"typeName":"publicationIDNumber","multiple":false,"typeClass":"primitive","value":"10.1093/jacamr/dlae085"},"publicationURL":{"typeName":"publicationURL","multiple":false,"typeClass":"primitive","value":"https://doi.org/10.1093/jacamr/dlae085"}}]},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"Bakker, Joke"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2023-10-27"}]},"dansDataVaultMetadata":{"displayName":"Data Vault Metadata","name":"dansDataVaultMetadata","fields":[{"typeName":"dansDataversePid","multiple":false,"typeClass":"primitive","value":"doi:10.34894/BG4ZS8"},{"typeName":"dansDataversePidVersion","multiple":false,"typeClass":"primitive","value":"1.2"},{"typeName":"dansBagId","multiple":false,"typeClass":"primitive","value":"urn:uuid:a7e27062-fdd9-49b0-9597-e2e02329a510"},{"typeName":"dansNbn","multiple":false,"typeClass":"primitive","value":"urn:nbn:nl:ui:13-b30257b7-ab83-4e3e-b35c-eba827aeaaff"}]}},"files":[{"description":"Flucuation test experiments - data for Ampicillin. ","label":"Amp.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":384094,"persistentId":"","filename":"Amp.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":5824281168,"description":"Flucuation test experiments - data for Ampicillin. ","storageIdentifier":"surf://store:18b860ba3ef-842bdcd6efb0","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"45736c293cbb8d746e86b6f119b150e91c542710"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"Flucuation test experiments - data for Ciprofloxacin. ","label":"Cipro.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":384095,"persistentId":"","filename":"Cipro.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":6706422997,"description":"Flucuation test experiments - data for Ciprofloxacin. ","storageIdentifier":"surf://store:18b861e5574-e9897391e1d1","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"29c140c1a5aa65cd76373ae1abd09a76a9c778d5"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"This folder contains the data from all flucuation test experiments (3 for Ciprofloxacin, 3 for Rifampicin, 5 for Ampicillin), it also includes the R-script necessary to analyze these data based on both CFU and CFP final population size measures.","label":"Fluctuation test.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":383990,"persistentId":"","filename":"Fluctuation test.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":36006,"description":"This folder contains the data from all flucuation test experiments (3 for Ciprofloxacin, 3 for Rifampicin, 5 for Ampicillin), it also includes the R-script necessary to analyze these data based on both CFU and CFP final population size measures.","storageIdentifier":"surf://store:18b85e98818-7d4dbb707ea3","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"1ecb754f2b4373162d69d450cc3b776d9958c05e"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"This folder contains the data and analysis script used to compare the growth rates at the two different temperatures.","label":"Growth rate.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":383988,"persistentId":"","filename":"Growth rate.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":203716,"description":"This folder contains the data and analysis script used to compare the growth rates at the two different temperatures.","storageIdentifier":"surf://store:18b85e98823-66d89fe5031f","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"2b5d15e702497206817fefb5837dc112f4eb6cf6"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"This folder contains the data and analysis script used to compare the plating efficiency at the two different temperatures.","label":"Plating efficiency.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":383989,"persistentId":"","filename":"Plating efficiency.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":100861801,"description":"This folder contains the data and analysis script used to compare the plating efficiency at the two different temperatures.","storageIdentifier":"surf://store:18b85e988b3-0b159d39ab47","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"cdd5e04f2137dd6d31975aef97199c2c75ba3bcb"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"Flucuation test experiments - data for Rifampicin. ","label":"Rif.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":384097,"persistentId":"","filename":"Rif.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":6841553380,"description":"Flucuation test experiments - data for Rifampicin. ","storageIdentifier":"surf://store:18b85fdfdfc-a13366489f4d","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"a0ea0210e4e35585ae6a7ee9ee36508ba806909f"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"This folder contains the raw sequencing data (subfolder Raw Data) and also includes the output of the Breseq analsysis including the reference genome used in the analysis (subfolder Breseq output). Samples are labeled by temperature (37/40) and by antibiotic A=Ampicillin, R=Rifampicin, C=ciprofloxacin, START indicates the unmutated strating strain. ","label":"Sequencing data-Breseq output.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":383991,"persistentId":"","filename":"Sequencing data-Breseq output.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":57145518,"description":"This folder contains the raw sequencing data (subfolder Raw Data) and also includes the output of the Breseq analsysis including the reference genome used in the analysis (subfolder Breseq output). Samples are labeled by temperature (37/40) and by antibiotic A=Ampicillin, R=Rifampicin, C=ciprofloxacin, START indicates the unmutated strating strain. ","storageIdentifier":"surf://store:18b85e98f95-fea6a578be3f","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"f6d90d7fc7e4c9e99178ab0bd2c43988d951bd70"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"Sequencing data: subfolder Raw Data - part 1. Samples are labeled by temperature (37/40) and by antibiotic A=Ampicillin, R=Rifampicin, C=ciprofloxacin, START indicates the unmutated strating strain. ","label":"Sequencing data-Raw data part 1.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":384093,"persistentId":"","filename":"Sequencing data-Raw data part 1.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":9088989605,"description":"Sequencing data: subfolder Raw Data - part 1. Samples are labeled by temperature (37/40) and by antibiotic A=Ampicillin, R=Rifampicin, C=ciprofloxacin, START indicates the unmutated strating strain. ","storageIdentifier":"surf://store:18b8649e13b-c5893ad98f6e","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"69b0c36828ea1186d8eb9238ab1f79c24a02ad4c"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"Sequencing data: subfolder Raw Data - part 2. Samples are labeled by temperature (37/40) and by antibiotic A=Ampicillin, R=Rifampicin, C=ciprofloxacin, START indicates the unmutated strating strain. ","label":"Sequencing data-Raw data part 2.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":384096,"persistentId":"","filename":"Sequencing data-Raw data part 2.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":9127099475,"description":"Sequencing data: subfolder Raw Data - part 2. Samples are labeled by temperature (37/40) and by antibiotic A=Ampicillin, R=Rifampicin, C=ciprofloxacin, START indicates the unmutated strating strain. ","storageIdentifier":"surf://store:18b86638047-6ea51c12c10e","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"29434ca479000b08c134488c2ba849c9d9ced2bd"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"Sequencing data: subfolder Raw Data - part 3. Samples are labeled by temperature (37/40) and by antibiotic A=Ampicillin, R=Rifampicin, C=ciprofloxacin, START indicates the unmutated strating strain. ","label":"Sequencing data-Raw data part 3.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":384098,"persistentId":"","filename":"Sequencing data-Raw data part 3.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":6540501059,"description":"Sequencing data: subfolder Raw Data - part 3. Samples are labeled by temperature (37/40) and by antibiotic A=Ampicillin, R=Rifampicin, C=ciprofloxacin, START indicates the unmutated strating strain. ","storageIdentifier":"surf://store:18b86310a78-fcacbc5b39c4","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"f8492d29f893872d2dbb9cc3ddbfe03ce4323dfa"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}},{"description":"This folder contains the experiments used to asses the temperature dependent efficacy of the three antibiotics.","label":"Temp dependent efficacy.zip","restricted":false,"version":1,"datasetVersionId":30477,"dataFile":{"id":383987,"persistentId":"","filename":"Temp dependent efficacy.zip","contentType":"application/zip","friendlyType":"ZIP Archive","filesize":3143599739,"description":"This folder contains the experiments used to asses the temperature dependent efficacy of the three antibiotics.","storageIdentifier":"surf://store:18b85e9a994-230845e31fed","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"ffef0e210788665d28355f28b8b613d10921ad41"},"tabularData":false,"creationDate":"2023-10-31","publicationDate":"2024-06-04","lastUpdateTime":"2025-04-10T12:38:31Z","fileAccessRequest":false}}]}}}