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Here, we show that chemically modified messenger RNA (cmRNA) encoding nerve growth factor (NGF) can stimulate neurogenic responses in vitro. NGF cmRNA was delivered to human bone marrow-derived mesenchymal stem cells using lipid carriers, leading to efficient NGF production. In co-culture, NGF-secreting stem cells induced differentiation of neural crest-derived PC-12 cells, as evidenced by increased neurite outgrowth and branching. Proteomic analyses revealed activation of neurogenesis-related pathways in both cell types. Notably, NGF cmRNA-transfected stem cells produced lower NGF levels than recombinant protein yet achieved comparable neurogenic effects, demonstrating the efficiency of the transcript-based approach. These findings establish NGF cmRNA as a potent inducer of neuronal differentiation and suggest its potential to enhance innervation during tissue repair, offering a promising approach for regenerative therapies, including bone healing."},"dsDescriptionDate":{"typeName":"dsDescriptionDate","multiple":false,"typeClass":"primitive","value":"2024-11-13"}}]},{"typeName":"subject","multiple":true,"typeClass":"controlledVocabulary","value":["Medicine, Health and Life Sciences"]},{"typeName":"keyword","multiple":true,"typeClass":"compound","value":[{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Innervation"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Bone"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Chemically modified mRNA"}},{"keywordValue":{"typeName":"keywordValue","multiple":false,"typeClass":"primitive","value":"Nerve growth factor"}}]},{"typeName":"language","multiple":true,"typeClass":"controlledVocabulary","value":["English"]},{"typeName":"depositor","multiple":false,"typeClass":"primitive","value":"Hebels, Dennie"},{"typeName":"dateOfDeposit","multiple":false,"typeClass":"primitive","value":"2024-11-13"}]},"dansDataVaultMetadata":{"displayName":"Data Vault Metadata","name":"dansDataVaultMetadata","fields":[{"typeName":"dansDataversePid","multiple":false,"typeClass":"primitive","value":"doi:10.34894/AX95AR"},{"typeName":"dansDataversePidVersion","multiple":false,"typeClass":"primitive","value":"1.0"},{"typeName":"dansBagId","multiple":false,"typeClass":"primitive","value":"urn:uuid:38dcdcdc-a28d-4917-9fcd-3a368ecc24c6"},{"typeName":"dansNbn","multiple":false,"typeClass":"primitive","value":"urn:nbn:nl:ui:13-99e441ca-0cce-4ebb-a803-d8c2bf9e9eaf"}]}},"files":[{"label":"Polain et al_Statistic report.docx","restricted":false,"version":1,"datasetVersionId":29876,"dataFile":{"id":458371,"persistentId":"","filename":"Polain et al_Statistic report.docx","contentType":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","friendlyType":"MS Word","filesize":662401,"storageIdentifier":"surf://store:19324ee5599-eee282e9381e","rootDataFileId":-1,"checksum":{"type":"SHA-1","value":"09cda798d775a33d9ab8c523776f394f4b5f7c4b"},"tabularData":false,"creationDate":"2024-11-13","publicationDate":"2026-10-01","lastUpdateTime":"2026-10-01T14:51:52Z","fileAccessRequest":true}}]}}}